human gdf 15 quantikine enzyme linked immunosorbent assay elisa kit  (R&D Systems)

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: A second generation of C3 humanized rats for preclinical evaluation of human C3 inhibitors

doi: 10.1016/j.bvth.2026.100138

Figure Lengend Snippet: The second-generation hC3 rats express human, but not rat C3 in their plasma and tissues, at levels higher than those in the first-generation hC3 rats. (A) Plasma from both human and rat samples were collected, and the presence of human (hC3) and rat C3 (rC3) were evaluated by western blots. Purified human C3 (hC3), rat C3 (rC3), human C3 depleted serum (hC3-dpl), and C3 knockout rat serum rat plasma (rC3-KO) were used as controls. Arrows pointed to the human or rat C3 band. (B) Plasma hC3 levels in the second-generation hC3 rats (both male and female) were evaluated using a human C3 ELISA kit (Hycult) and compared to the levels in the first-generation hC3 rat plasma. (C) Total RNA was extracted from the liver, retina, spleen, kidney, and brain of WT, second-generation, and first-generation hC3 rats, and then semiquantitative reverse transcription PCR was used to detect and quantitate levels of human C3 (hC3) transcripts using the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as internal controls. 1st gen, first generation; 2nd gen, second generation.

Article Snippet: Blood levels of human C3 protein were measured in the identified human C3 Tg rats using an enzyme-linked immunosorbent assay (ELISA) kit (Hycult, catalog no. HK366) to identify the founder rats with close-to-physiological expression levels of human C3.

Techniques: Clinical Proteomics, Western Blot, Purification, Knock-Out, Enzyme-linked Immunosorbent Assay, Reverse Transcription

Journal: Frontiers in Physiology

Article Title: The possible mechanisms linking chronic obstructive pulmonary disease and coronary atherosclerosis based on coronary computed tomography angiography and animal experiments

doi: 10.3389/fphys.2026.1688832

Figure Lengend Snippet: Oxidative stress response and the KEAP1–NRF2 pathway levels are linked to CAS in COPD patients. (A) ELISA detection of KEAP1 and NRF2 levels in the serum of patients. (B) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of patients determined via RT-qPCR. (C) Levels of ROS and MDA, and activities of SOD and catalase in the serum of patients determined using kits. The CAS group was used as a normalization control. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests with corrections for multiple comparisons. CAS, coronary atherosclerosis; COPD, chronic obstructive pulmonary disease; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.

Article Snippet: Serum KEAP1 and Nrf2 levels were measured using a human KEAP1 enzyme-linked immunosorbent assay (ELISA) kit (EH4240, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China) and a human Nrf2 ELISA kit (abs551899, Absin Bioscience Inc., Shanghai, China) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Control

Journal: Frontiers in Physiology

Article Title: The possible mechanisms linking chronic obstructive pulmonary disease and coronary atherosclerosis based on coronary computed tomography angiography and animal experiments

doi: 10.3389/fphys.2026.1688832

Figure Lengend Snippet: KEAP1–NRF2-mediated oxidative stress participates in the occurrence of COPD combined with CAS in mice. (A) PFT for FRC, RI, Cdyn, and MV in mice. (B) The histopathological changes of lung tissues observed using H&E staining. (C) Serum lipid levels in mice measured via ELISA. (D) KEAP1, NRF2, NQO1, and HO-1 levels in the serum of mice determined via RT-qPCR. (E) Levels of ROS and MDA, and activities of SOD and catalase in the lung tissue of mice measured using kits. (F) Representative Western blotting of KEAP1, NRF2, NQO1, and HO-1 proteins in the lung tissue of mice and the quantification data. n = 6 mice for each treatment. Data among multiple groups were compared using one-way ANOVA, followed by Tukey’s post-hoc tests for multiple comparisons. COPD, chronic obstructive pulmonary disease; CAS, coronary atherosclerosis; PFT, pulmonary function test; FRC, functional residual capacity; RI, resistance index; Cdyn, dynamic compliance; MV, minute ventilation; ROS, reactive oxygen species; MDA, malondialdehyde; SOD, superoxide dismutase.

Article Snippet: Serum KEAP1 and Nrf2 levels were measured using a human KEAP1 enzyme-linked immunosorbent assay (ELISA) kit (EH4240, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China) and a human Nrf2 ELISA kit (abs551899, Absin Bioscience Inc., Shanghai, China) according to the manufacturer’s instructions.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Functional Assay

Journal: The Journal of Nutrition, Health & Aging

Article Title: Association of growth differentiation factor-15 with muscle wasting and anemia in chronic kidney disease

doi: 10.1016/j.jnha.2026.100835

Figure Lengend Snippet: Factors associated with GDF-15. Univariate analysis of the correlations of GDF-15 with the (a) estimated glomerular filtration rate (eGFR), (b) ln urine protein-to-creatinine ratio (UPCR), (c) ln N-terminal pro-B-type natriuretic peptide (NT-proBNP), (d) hemoglobin, (e) ln interleukin-6 (IL-6), (f) ln tumor necrosis factor-α (TNF-α), (g) albumin, and (h) lean tissue index.

Article Snippet: Plasma levels of GDF-15 were quantified using the Human GDF-15 Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems, Minneapolis, MN, USA), with intra- and inter-assay coefficients of variation <5%.

Techniques: Filtration

Journal: The Journal of Nutrition, Health & Aging

Article Title: Association of growth differentiation factor-15 with muscle wasting and anemia in chronic kidney disease

doi: 10.1016/j.jnha.2026.100835

Figure Lengend Snippet: Kaplan–Meier curves by growth differentiation factor-15 (GDF-15) median. Participants with GDF-15 concentrations above the median had a higher cumulative incidence of all-cause mortality than those with lower concentrations (log-rank P < 0.001). Numbers at risk are shown below the plot.

Article Snippet: Plasma levels of GDF-15 were quantified using the Human GDF-15 Quantikine enzyme-linked immunosorbent assay (ELISA) Kit (R&D Systems, Minneapolis, MN, USA), with intra- and inter-assay coefficients of variation <5%.

Techniques: